Application of high resolution 2d-spr imager to living cell-based allergen sensing

M. Horii, H. Shinohara, Y. Iribe, M. Suzuki

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

5 Scopus citations

Abstract

Newly developed two-dimensional surface plasmon resonance (2D-SPR) sensor system can obtain 2D image of refractive index difference on the surface of a gold thm film in 1 urn resolution. Here, we examined to observe the allergenic response of a mast cell model, rat basophilic leukaemia cell (RBL-2H3) by using the 2D-SPR imaging system after pres e n i l a t i o n with 0.1 ug/mL anti-dimtrophenyl (DNP) immunoglobulin E (IgE). Response of individual cell was successfully observed upon the stimulation with DNP-modified bovine serum albumin (DNP-BSA) using the 2D-SPR system. Furthermore, multi-channel measurement was performed for sensitized and non-sensitized cells in a polydimethylsiloxane (PDMS) well-array sheet.

Original languageEnglish
Title of host publicationProceedings of the 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007
EditorsJean-Louis Viovy, Patrick Tabeling, Stephanie Descroix, Laurent Malaquin
PublisherChemical and Biological Microsystems Society
Pages451-453
Number of pages3
ISBN (Electronic)9780979806407
StatePublished - 2007
Event11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007 - Paris, France
Duration: 2007/10/072007/10/11

Publication series

NameProceedings of the 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007

Conference

Conference11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007
Country/TerritoryFrance
CityParis
Period2007/10/072007/10/11

Keywords

  • 2D-SPR
  • Living cell imaging
  • Mast cell response
  • Multi channel sensing

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

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