Apoptosis induced by the sonomechanical effects of low intensity pulsed ultrasound in a human leukemia cell line

Loreto B. Feril; Takashi Kondo; Zheng Guo Cui; Yoshiaki Tabuchi; Qing Li Zhao; Hidetaka Ando; Takuro Misaki; Hideki Yoshikawa; Shin Ichiro Umemura

doi:10.1016/j.canlet.2004.08.034

Apoptosis induced by the sonomechanical effects of low intensity pulsed ultrasound in a human leukemia cell line

Loreto B. Feril, Takashi Kondo^*, Zheng Guo Cui, Yoshiaki Tabuchi, Qing Li Zhao, Hidetaka Ando, Takuro Misaki, Hideki Yoshikawa, Shin Ichiro Umemura

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

136 Scopus citations

Abstract

To obtain an optimal condition for ultrasound (US)-induced apoptosis that could be useful for cancer therapy, we applied low intensity pulsed US to sonicate U937 cells in vitro. Cells were then incubated at different time intervals before measuring apoptosis. The apoptosis was assessed by DNA fragmentation and phosphatidylserine externalization. The pattern of the decrease in mitochondrial membrane potential was determined by flow cytometry. Optimal apoptosis (70.0±13.8%) with minimal lysis was attained with 1 MHz ultrasound 0.3 W/cm², 10% duty factor at 100 Hz for 1 min) at 12 h after sonication. Lack of US-induced free radical detection and absence of Heme oxygenase-1, an intracellular oxidative stress marker, up-regulation in cells, suggest that sonomechanical, not sonochemical, effects are the main mechanism involved.

Original language	English
Pages (from-to)	145-152
Number of pages	8
Journal	Cancer Letters
Volume	221
Issue number	2
DOIs	https://doi.org/10.1016/j.canlet.2004.08.034
State	Published - 2005/04/28

Keywords

Apoptosis
Low intensity ultrasound
Sonomechanical effects

ASJC Scopus subject areas

Oncology
Cancer Research

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.canlet.2004.08.034

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title = "Apoptosis induced by the sonomechanical effects of low intensity pulsed ultrasound in a human leukemia cell line",

abstract = "To obtain an optimal condition for ultrasound (US)-induced apoptosis that could be useful for cancer therapy, we applied low intensity pulsed US to sonicate U937 cells in vitro. Cells were then incubated at different time intervals before measuring apoptosis. The apoptosis was assessed by DNA fragmentation and phosphatidylserine externalization. The pattern of the decrease in mitochondrial membrane potential was determined by flow cytometry. Optimal apoptosis (70.0±13.8%) with minimal lysis was attained with 1 MHz ultrasound 0.3 W/cm2, 10% duty factor at 100 Hz for 1 min) at 12 h after sonication. Lack of US-induced free radical detection and absence of Heme oxygenase-1, an intracellular oxidative stress marker, up-regulation in cells, suggest that sonomechanical, not sonochemical, effects are the main mechanism involved.",

keywords = "Apoptosis, Low intensity ultrasound, Sonomechanical effects",

author = "Feril, {Loreto B.} and Takashi Kondo and Cui, {Zheng Guo} and Yoshiaki Tabuchi and Zhao, {Qing Li} and Hidetaka Ando and Takuro Misaki and Hideki Yoshikawa and Umemura, {Shin Ichiro}",

note = "Funding Information: This study is supported in part by the Grant in Aid for Scientific Research on Priority Areas {\textcopyright} (12217049) from the Ministry of Education, Culture, Sports, Sciences and Technology, Japan; in part by the Research and Development Committee Program of the Japan Society of Ultrasonics in Medicine; in part by the 21st Century COE Program of the Toyama Medical and Pharmaceutical University, Japan and also in part by the International Association for the Sensitization of Cancer Treatment (IASCT).",

year = "2005",

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pages = "145--152",

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AU - Feril, Loreto B.

AU - Kondo, Takashi

AU - Cui, Zheng Guo

AU - Tabuchi, Yoshiaki

AU - Zhao, Qing Li

AU - Ando, Hidetaka

AU - Misaki, Takuro

AU - Yoshikawa, Hideki

AU - Umemura, Shin Ichiro

N1 - Funding Information: This study is supported in part by the Grant in Aid for Scientific Research on Priority Areas © (12217049) from the Ministry of Education, Culture, Sports, Sciences and Technology, Japan; in part by the Research and Development Committee Program of the Japan Society of Ultrasonics in Medicine; in part by the 21st Century COE Program of the Toyama Medical and Pharmaceutical University, Japan and also in part by the International Association for the Sensitization of Cancer Treatment (IASCT).

PY - 2005/4/28

Y1 - 2005/4/28

N2 - To obtain an optimal condition for ultrasound (US)-induced apoptosis that could be useful for cancer therapy, we applied low intensity pulsed US to sonicate U937 cells in vitro. Cells were then incubated at different time intervals before measuring apoptosis. The apoptosis was assessed by DNA fragmentation and phosphatidylserine externalization. The pattern of the decrease in mitochondrial membrane potential was determined by flow cytometry. Optimal apoptosis (70.0±13.8%) with minimal lysis was attained with 1 MHz ultrasound 0.3 W/cm2, 10% duty factor at 100 Hz for 1 min) at 12 h after sonication. Lack of US-induced free radical detection and absence of Heme oxygenase-1, an intracellular oxidative stress marker, up-regulation in cells, suggest that sonomechanical, not sonochemical, effects are the main mechanism involved.

AB - To obtain an optimal condition for ultrasound (US)-induced apoptosis that could be useful for cancer therapy, we applied low intensity pulsed US to sonicate U937 cells in vitro. Cells were then incubated at different time intervals before measuring apoptosis. The apoptosis was assessed by DNA fragmentation and phosphatidylserine externalization. The pattern of the decrease in mitochondrial membrane potential was determined by flow cytometry. Optimal apoptosis (70.0±13.8%) with minimal lysis was attained with 1 MHz ultrasound 0.3 W/cm2, 10% duty factor at 100 Hz for 1 min) at 12 h after sonication. Lack of US-induced free radical detection and absence of Heme oxygenase-1, an intracellular oxidative stress marker, up-regulation in cells, suggest that sonomechanical, not sonochemical, effects are the main mechanism involved.

KW - Apoptosis

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KW - Sonomechanical effects

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Apoptosis induced by the sonomechanical effects of low intensity pulsed ultrasound in a human leukemia cell line

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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