TY - JOUR
T1 - Antiproliferative activity of the Netherlands propolis and its active principles in cancer cell lines
AU - Banskota, Arjun H.
AU - Nagaoka, Takema
AU - Sumioka, Lucia Yoshie
AU - Tezuka, Yasuhiro
AU - Awale, Suresh
AU - Midorikawa, Kiyoshi
AU - Matsushige, Katsumichi
AU - Kadota, Shigetoshi
PY - 2002
Y1 - 2002
N2 - The MeOH extract of the Netherlands propolis showed promising antiproliferative activity toward highly liver-metastatic murine colon 26-L5 carcinoma with an EC50 value of 3.5 μ g/ml. Further, antiproliferative activity-guided purification of the MeOH extract led us to isolate four flavonoids (1-4), seven cinnamic acid derivatives (5-11) and two new glycerol derivatives (12, 13), whose structures were elucidated on the basis of spectral analysis. The isolated compounds were tested for their antiproliferative activity against murine colon 26-L5, murine B16-BL6 melanoma, human HT-1080 fibrosarcoma and human lung A549 adenocarcinoma cell lines. The benzyl (9), phenethyl (10) and cinnamyl caffeates (11) possessed potent antiproliferative activities with EC50 values of 0.288, 1.76 and 0.114 μM, respectively, toward colon 26-L5 carcinoma. These caffeates were considered to be active constituents of the Netherlands propolis in their antiproliferative activity. The antioxidative activity of these caffeates may play an important role in their antiproliferative activities.
AB - The MeOH extract of the Netherlands propolis showed promising antiproliferative activity toward highly liver-metastatic murine colon 26-L5 carcinoma with an EC50 value of 3.5 μ g/ml. Further, antiproliferative activity-guided purification of the MeOH extract led us to isolate four flavonoids (1-4), seven cinnamic acid derivatives (5-11) and two new glycerol derivatives (12, 13), whose structures were elucidated on the basis of spectral analysis. The isolated compounds were tested for their antiproliferative activity against murine colon 26-L5, murine B16-BL6 melanoma, human HT-1080 fibrosarcoma and human lung A549 adenocarcinoma cell lines. The benzyl (9), phenethyl (10) and cinnamyl caffeates (11) possessed potent antiproliferative activities with EC50 values of 0.288, 1.76 and 0.114 μM, respectively, toward colon 26-L5 carcinoma. These caffeates were considered to be active constituents of the Netherlands propolis in their antiproliferative activity. The antioxidative activity of these caffeates may play an important role in their antiproliferative activities.
KW - Antiproliferative activity
KW - Benzyl caffeate
KW - Cinnamyl caffeate
KW - DPPH radical scavenging activity
KW - Phenethyl caffeate
KW - Propolis
UR - http://www.scopus.com/inward/record.url?scp=0036124548&partnerID=8YFLogxK
U2 - 10.1016/S0378-8741(02)00022-3
DO - 10.1016/S0378-8741(02)00022-3
M3 - 学術論文
C2 - 11891088
AN - SCOPUS:0036124548
SN - 0378-8741
VL - 80
SP - 67
EP - 73
JO - Journal of Ethnopharmacology
JF - Journal of Ethnopharmacology
IS - 1
ER -
