Anion and cation channels in the basolateral membrane of rabbit parietal cells

Hideki Sakai, Yasumobu Okada, Magotoshi Morii, Noriaki Takeguchi*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

38 Scopus citations

Abstract

Ion channels in the basolateral membrane of rabbit parietal cells in isolated gastric glands were studied by the patch clamp technique. Whole-cell current-clamp recordings showed that the membrane potential (Em) changed systematically as a function of the chloride concentrations of the basolateral bathing solution ([Cl-]0), and of the pipette (intracellular) solution. The relationship between Em and [Cl-]0 was not affected by additions of histamine, dibutyryl-cAMP, 4-acetoamido-4′-isothiocyanostilbene-2,2′-disulfonic acid and diphenylamine-2-carboxylate. The whole-cell Cl- conductance was insensitive to voltage. In cell-attached and cell-free patch membranes, however, single Cl- channel opening events could not be observed. The value of Em depended little on the basolateral K+ concentration, but inward-rectifier K+ currents were observed in the whole-cell configuration, activated by hyperpolarizing pulses and inhibited by extracellular Ba2+. In cell-attached and cell-free patches, openings of single inward-rectifier K+ channels and non-selective cation channels were infrequently recorded. Neither cAMP nor Ca2+ activated these cation channels. The single K+ channel conductance was about 230 pS under the symmetrical high K+ conditions and was inhibited by intracellular tetraethylammonium ions (TEA). The non-selective cation channel had a voltage-independent single conductance of 22 pS and was not inhibited by TEA.

Original languageEnglish
Pages (from-to)185-192
Number of pages8
JournalPflugers Archiv European Journal of Physiology
Volume414
Issue number2
DOIs
StatePublished - 1989/06

Keywords

  • Basolateral membrane
  • Cl channel
  • K channel
  • Parietal cell
  • Patch clamp

ASJC Scopus subject areas

  • Physiology
  • Clinical Biochemistry
  • Physiology (medical)

Fingerprint

Dive into the research topics of 'Anion and cation channels in the basolateral membrane of rabbit parietal cells'. Together they form a unique fingerprint.

Cite this