Analysis of dissociated single neurons by simple and semi-quantitative RT-PCR (Reverse transcription and polymerase chain reaction)

Tsugunobu Andoh; Chihiro Tohda; Yasushi Kuraishi

doi:10.1254/jjp.74.121

Analysis of dissociated single neurons by simple and semi-quantitative RT-PCR (Reverse transcription and polymerase chain reaction)

Tsugunobu Andoh, Chihiro Tohda, Yasushi Kuraishi^*

^*Corresponding author for this work

Section of Neuromedical Science, Division of Bioscience, Institute of Natural Medicine

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

We have developed a simple and semi-quantitative method for mRNA determination in single cells using the reverse transcription and polymerase chain reaction (RT-PCR). The distinct features of this method are the highly efficient RNA harvest from whole dissociated cells and the ability to perform all RT procedures in one tube that allowed semi-quantitative determination of mRNA in dissociated cells. This method revealed that histamine H₁-receptor mRNA was highly expressed in 5/28 small and 1/26 large dorsal root ganglion neurons of the mouse.

Original language	English
Pages (from-to)	121-123
Number of pages	3
Journal	Japanese Journal of Pharmacology
Volume	74
Issue number	1
DOIs	https://doi.org/10.1254/jjp.74.121
State	Published - 1997/05

Keywords

Histamine H-receptor mRNA
Primary sensory neuron
Single whole-cell PCR

ASJC Scopus subject areas

Pharmacology

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10.1254/jjp.74.121

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abstract = "We have developed a simple and semi-quantitative method for mRNA determination in single cells using the reverse transcription and polymerase chain reaction (RT-PCR). The distinct features of this method are the highly efficient RNA harvest from whole dissociated cells and the ability to perform all RT procedures in one tube that allowed semi-quantitative determination of mRNA in dissociated cells. This method revealed that histamine H1-receptor mRNA was highly expressed in 5/28 small and 1/26 large dorsal root ganglion neurons of the mouse.",

keywords = "Histamine H-receptor mRNA, Primary sensory neuron, Single whole-cell PCR",

author = "Tsugunobu Andoh and Chihiro Tohda and Yasushi Kuraishi",

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AU - Andoh, Tsugunobu

AU - Tohda, Chihiro

AU - Kuraishi, Yasushi

PY - 1997/5

Y1 - 1997/5

N2 - We have developed a simple and semi-quantitative method for mRNA determination in single cells using the reverse transcription and polymerase chain reaction (RT-PCR). The distinct features of this method are the highly efficient RNA harvest from whole dissociated cells and the ability to perform all RT procedures in one tube that allowed semi-quantitative determination of mRNA in dissociated cells. This method revealed that histamine H1-receptor mRNA was highly expressed in 5/28 small and 1/26 large dorsal root ganglion neurons of the mouse.

AB - We have developed a simple and semi-quantitative method for mRNA determination in single cells using the reverse transcription and polymerase chain reaction (RT-PCR). The distinct features of this method are the highly efficient RNA harvest from whole dissociated cells and the ability to perform all RT procedures in one tube that allowed semi-quantitative determination of mRNA in dissociated cells. This method revealed that histamine H1-receptor mRNA was highly expressed in 5/28 small and 1/26 large dorsal root ganglion neurons of the mouse.

KW - Histamine H-receptor mRNA

KW - Primary sensory neuron

KW - Single whole-cell PCR

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JF - Japanese Journal of Pharmacology

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Analysis of dissociated single neurons by simple and semi-quantitative RT-PCR (Reverse transcription and polymerase chain reaction)

Abstract

Keywords

ASJC Scopus subject areas

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