TY - JOUR
T1 - Action of neurotropin on rat hypothalamic neurons in tissue slices
AU - Nakamura, Kiyomi
AU - Ono, Taketoshi
AU - Nishijo, Hisao
AU - Tamura, Ryoi
N1 - Funding Information:
We thank Prof. A. Simpson, Showa University, for reviewing the manuscript,N ippon Zoki Co. for kindly providing Neurotropin, and Mrs. M. Yamazaki and Miss A. Hatayamaf or typing. This work was supported in part by the Japanese Ministry of Education, Science and Culture, Grant-in-Aid for Scientific Research, 60440028.
PY - 1990/6
Y1 - 1990/6
N2 - To clarify some of the actions of Neurotropin (NSP), intra- and extracellular measurements were made of 140 paraventricular (PVN) and 48 ventromedial nucleus (VMH) neurons in rat hypothalamic tissue slices in vitro while perfusing NSP, an extract from the inflamed skin of rabbits inoculated with vaccinia virus, through the recording chamber. NSP mostly decreased activity in PVN neurons (inhibition, 46; excitation, 27; excitation-inhibition, 5; no response, 62), and mostly increased it in VMH neurons (excitation, 12; inhibition, 3; no response, 33). Inhibition of PVN neurons by NSP was due to hyperpolarization with no change in membrane conductance. Since ouabain antagonized the NSP-induced inhibition of PVN neurons, the effect was probably due to activation of the sodium pump. Activity of some NSP-responsive PVN neurons was increased by increase of extracellular osmolarity, and activity of some NSP-responsive VMH neurons was increased by glucose application. The results suggest central modulation of autonomic or neuroendocrinological function by distinct NSP influence on PVN and VMH neural activity.
AB - To clarify some of the actions of Neurotropin (NSP), intra- and extracellular measurements were made of 140 paraventricular (PVN) and 48 ventromedial nucleus (VMH) neurons in rat hypothalamic tissue slices in vitro while perfusing NSP, an extract from the inflamed skin of rabbits inoculated with vaccinia virus, through the recording chamber. NSP mostly decreased activity in PVN neurons (inhibition, 46; excitation, 27; excitation-inhibition, 5; no response, 62), and mostly increased it in VMH neurons (excitation, 12; inhibition, 3; no response, 33). Inhibition of PVN neurons by NSP was due to hyperpolarization with no change in membrane conductance. Since ouabain antagonized the NSP-induced inhibition of PVN neurons, the effect was probably due to activation of the sodium pump. Activity of some NSP-responsive PVN neurons was increased by increase of extracellular osmolarity, and activity of some NSP-responsive VMH neurons was increased by glucose application. The results suggest central modulation of autonomic or neuroendocrinological function by distinct NSP influence on PVN and VMH neural activity.
KW - Autonomic nervous system
KW - Neural activity
KW - Neurotropin
KW - Paraventricular nucleus
KW - Ventromedial nucleus
UR - http://www.scopus.com/inward/record.url?scp=0025290865&partnerID=8YFLogxK
U2 - 10.1016/0361-9230(90)90144-O
DO - 10.1016/0361-9230(90)90144-O
M3 - 学術論文
C2 - 2372698
AN - SCOPUS:0025290865
SN - 0361-9230
VL - 24
SP - 811
EP - 817
JO - Brain Research Bulletin
JF - Brain Research Bulletin
IS - 6
ER -
