TY - JOUR
T1 - Acinetobacter sp. Ud-4 efficiently degrades both edible and mineral oils
T2 - Isolation and characterization
AU - Tanaka, Daisuke
AU - Takashima, Miyuki
AU - Mizuta, Asako
AU - Tanaka, Shunsuke
AU - Sakatoku, Akihiro
AU - Nishikawa, Atsushi
AU - Osawa, Tsutomu
AU - Noguchi, Munenori
AU - Aizawa, Shin Ichi
AU - Nakamura, Shogo
N1 - Funding Information:
This work was supported in part by a Grant-in-Aid from the Ministry of Education, Culture, Sports, Science and Technology of Japan (No. 19510082).
PY - 2010/3
Y1 - 2010/3
N2 - A novel Acinetobacter strain, Ud-4, possessing a strong capacity to degrade edible, lubricating, and heavy oil was isolated from seawater in a fishing port located in Toyama, Japan. It was identified by morphological and physiological analyses and 16S rDNA sequencing. This strain could utilize five types of edible oils (canola oil, olive oil, sesame oil, soybean oil, and lard), lubricating oil, and C-heavy oil as the sole carbon source for growth in M9 medium. The strain grew well and heavily degraded edible oils in Luria-Bertani medium during a 7-day culture at 25°C; it also degraded all kinds of oils in artificial seawater medium for marine bacteria. Furthermore, this strain was capable of degrading almost all C10-C25 n-alkanes in C-heavy oil during a 4-week culture. Oligonucleotide primers specific to two catabolic genes involved in the degradation of n-alkanes (Acinetobacter sp. alkM) and triglyceride (Acinetobacter sp. lipA) allowed amplification of these genes in strain Ud-4. To our knowledge, this is the first report on the isolation of a bacterium that can efficiently degrade both edible and mineral oils.
AB - A novel Acinetobacter strain, Ud-4, possessing a strong capacity to degrade edible, lubricating, and heavy oil was isolated from seawater in a fishing port located in Toyama, Japan. It was identified by morphological and physiological analyses and 16S rDNA sequencing. This strain could utilize five types of edible oils (canola oil, olive oil, sesame oil, soybean oil, and lard), lubricating oil, and C-heavy oil as the sole carbon source for growth in M9 medium. The strain grew well and heavily degraded edible oils in Luria-Bertani medium during a 7-day culture at 25°C; it also degraded all kinds of oils in artificial seawater medium for marine bacteria. Furthermore, this strain was capable of degrading almost all C10-C25 n-alkanes in C-heavy oil during a 4-week culture. Oligonucleotide primers specific to two catabolic genes involved in the degradation of n-alkanes (Acinetobacter sp. alkM) and triglyceride (Acinetobacter sp. lipA) allowed amplification of these genes in strain Ud-4. To our knowledge, this is the first report on the isolation of a bacterium that can efficiently degrade both edible and mineral oils.
UR - http://www.scopus.com/inward/record.url?scp=76949097891&partnerID=8YFLogxK
U2 - 10.1007/s00284-009-9525-5
DO - 10.1007/s00284-009-9525-5
M3 - 学術論文
C2 - 19847483
AN - SCOPUS:76949097891
SN - 0343-8651
VL - 60
SP - 203
EP - 209
JO - Current Microbiology
JF - Current Microbiology
IS - 3
ER -
