Ablation of Elovl6 protects pancreatic islets from high-fat diet-induced impairment of insulin secretion

Nie Tang; Takashi Matsuzaka; Marii Suzuki; Yuta Nakano; Hui Zao; Tomotaka Yokoo; Noriko Suzuki-Kemuriyama; Motoko Kuba; Yuka Okajima; Yoshinori Takeuchi; Kazuto Kobayashi; Hitoshi Iwasaki; Shigeru Yatoh; Akimitsu Takahashi; Hiroaki Suzuki; Hirohito Sone; Masako Shimada; Yoshimi Nakagawa; Naoya Yahagi; Nobuhiro Yamada; Hitoshi Shimano

doi:10.1016/j.bbrc.2014.05.113

Ablation of Elovl6 protects pancreatic islets from high-fat diet-induced impairment of insulin secretion

Nie Tang, Takashi Matsuzaka^*, Marii Suzuki, Yuta Nakano, Hui Zao, Tomotaka Yokoo, Noriko Suzuki-Kemuriyama, Motoko Kuba, Yuka Okajima, Yoshinori Takeuchi, Kazuto Kobayashi, Hitoshi Iwasaki, Shigeru Yatoh, Akimitsu Takahashi, Hiroaki Suzuki, Hirohito Sone, Masako Shimada, Yoshimi Nakagawa, Naoya Yahagi, Nobuhiro YamadaHitoshi Shimano

^*Corresponding author for this work

Complex Biosystem Research, Institute of Natural Medicine

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

ELOVL family member 6, elongation of very long-chain fatty acids (Elovl6) is a microsomal enzyme that regulates the elongation of C12-16 saturated and monounsaturated fatty acids and is related to the development of obesity-induced insulin resistance via the modification of the fatty acid composition. In this study, we investigated the role of systemic Elovl6 in the pancreatic islet and β-cell function. Elovl6 is expressed in both islets and β-cell lines. In mice fed with chow, islets of the Elovl6^-/- mice displayed normal architecture and β-cell mass compared with those of the wild-type mice. However, when fed a high-fat, high-sucrose (HFHS) diet, the islet hypertrophy in response to insulin resistance observed in normal mice was attenuated and glucose-stimulated insulin secretion (GSIS) increased in the islets of Elovl6^-/- mice compared with those of the wild-type mice. Enhanced GSIS in the HFHS Elovl6^-/- islets was associated with an increased ATP/ADP ratio and the suppression of ATF-3 expression. Our findings suggest that Elovl6 could be involved in insulin secretory capacity per β-cell and diabetes.

Original language	English
Pages (from-to)	318-323
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	450
Issue number	1
DOIs	https://doi.org/10.1016/j.bbrc.2014.05.113
State	Published - 2014/07/18

Keywords

Fatty acid
Insulin
Islet
Obesity
Type 2 diabetes
β-cell

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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Tang, N., Matsuzaka, T., Suzuki, M., Nakano, Y., Zao, H., Yokoo, T., Suzuki-Kemuriyama, N., Kuba, M., Okajima, Y., Takeuchi, Y., Kobayashi, K., Iwasaki, H., Yatoh, S., Takahashi, A., Suzuki, H., Sone, H., Shimada, M., Nakagawa, Y., Yahagi, N., ... Shimano, H. (2014). Ablation of Elovl6 protects pancreatic islets from high-fat diet-induced impairment of insulin secretion. Biochemical and Biophysical Research Communications, 450(1), 318-323. https://doi.org/10.1016/j.bbrc.2014.05.113

@article{dc1f3880bc3c4ef18d06ff3dd45ad6d8,

title = "Ablation of Elovl6 protects pancreatic islets from high-fat diet-induced impairment of insulin secretion",

abstract = "ELOVL family member 6, elongation of very long-chain fatty acids (Elovl6) is a microsomal enzyme that regulates the elongation of C12-16 saturated and monounsaturated fatty acids and is related to the development of obesity-induced insulin resistance via the modification of the fatty acid composition. In this study, we investigated the role of systemic Elovl6 in the pancreatic islet and β-cell function. Elovl6 is expressed in both islets and β-cell lines. In mice fed with chow, islets of the Elovl6-/- mice displayed normal architecture and β-cell mass compared with those of the wild-type mice. However, when fed a high-fat, high-sucrose (HFHS) diet, the islet hypertrophy in response to insulin resistance observed in normal mice was attenuated and glucose-stimulated insulin secretion (GSIS) increased in the islets of Elovl6-/- mice compared with those of the wild-type mice. Enhanced GSIS in the HFHS Elovl6-/- islets was associated with an increased ATP/ADP ratio and the suppression of ATF-3 expression. Our findings suggest that Elovl6 could be involved in insulin secretory capacity per β-cell and diabetes.",

keywords = "Fatty acid, Insulin, Islet, Obesity, Type 2 diabetes, β-cell",

author = "Nie Tang and Takashi Matsuzaka and Marii Suzuki and Yuta Nakano and Hui Zao and Tomotaka Yokoo and Noriko Suzuki-Kemuriyama and Motoko Kuba and Yuka Okajima and Yoshinori Takeuchi and Kazuto Kobayashi and Hitoshi Iwasaki and Shigeru Yatoh and Akimitsu Takahashi and Hiroaki Suzuki and Hirohito Sone and Masako Shimada and Yoshimi Nakagawa and Naoya Yahagi and Nobuhiro Yamada and Hitoshi Shimano",

note = "Funding Information: The authors thank Chizuko Fukui, Katsuko Okubo, and Yuko Tamai for their technical assistance, and Enago ( www.enago.jp ) for the English language review. This work was supported by the Japan Foundation for Applied Enzymology (to T.M.) and Grant-in-Aid for Scientific Research 24390230 (to H.S.) from the Ministry of Science, Education, Culture, and Technology of Japan . ",

year = "2014",

month = jul,

day = "18",

doi = "10.1016/j.bbrc.2014.05.113",

language = "英語",

volume = "450",

pages = "318--323",

journal = "Biochemical and Biophysical Research Communications",

issn = "0006-291X",

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Tang, N, Matsuzaka, T, Suzuki, M, Nakano, Y, Zao, H, Yokoo, T, Suzuki-Kemuriyama, N, Kuba, M, Okajima, Y, Takeuchi, Y, Kobayashi, K, Iwasaki, H, Yatoh, S, Takahashi, A, Suzuki, H, Sone, H, Shimada, M, Nakagawa, Y, Yahagi, N, Yamada, N & Shimano, H 2014, 'Ablation of Elovl6 protects pancreatic islets from high-fat diet-induced impairment of insulin secretion', Biochemical and Biophysical Research Communications, vol. 450, no. 1, pp. 318-323. https://doi.org/10.1016/j.bbrc.2014.05.113

TY - JOUR

T1 - Ablation of Elovl6 protects pancreatic islets from high-fat diet-induced impairment of insulin secretion

AU - Tang, Nie

AU - Matsuzaka, Takashi

AU - Suzuki, Marii

AU - Nakano, Yuta

AU - Zao, Hui

AU - Yokoo, Tomotaka

AU - Suzuki-Kemuriyama, Noriko

AU - Kuba, Motoko

AU - Okajima, Yuka

AU - Takeuchi, Yoshinori

AU - Kobayashi, Kazuto

AU - Iwasaki, Hitoshi

AU - Yatoh, Shigeru

AU - Takahashi, Akimitsu

AU - Suzuki, Hiroaki

AU - Sone, Hirohito

AU - Shimada, Masako

AU - Nakagawa, Yoshimi

AU - Yahagi, Naoya

AU - Yamada, Nobuhiro

AU - Shimano, Hitoshi

N1 - Funding Information: The authors thank Chizuko Fukui, Katsuko Okubo, and Yuko Tamai for their technical assistance, and Enago ( www.enago.jp ) for the English language review. This work was supported by the Japan Foundation for Applied Enzymology (to T.M.) and Grant-in-Aid for Scientific Research 24390230 (to H.S.) from the Ministry of Science, Education, Culture, and Technology of Japan .

PY - 2014/7/18

Y1 - 2014/7/18

N2 - ELOVL family member 6, elongation of very long-chain fatty acids (Elovl6) is a microsomal enzyme that regulates the elongation of C12-16 saturated and monounsaturated fatty acids and is related to the development of obesity-induced insulin resistance via the modification of the fatty acid composition. In this study, we investigated the role of systemic Elovl6 in the pancreatic islet and β-cell function. Elovl6 is expressed in both islets and β-cell lines. In mice fed with chow, islets of the Elovl6-/- mice displayed normal architecture and β-cell mass compared with those of the wild-type mice. However, when fed a high-fat, high-sucrose (HFHS) diet, the islet hypertrophy in response to insulin resistance observed in normal mice was attenuated and glucose-stimulated insulin secretion (GSIS) increased in the islets of Elovl6-/- mice compared with those of the wild-type mice. Enhanced GSIS in the HFHS Elovl6-/- islets was associated with an increased ATP/ADP ratio and the suppression of ATF-3 expression. Our findings suggest that Elovl6 could be involved in insulin secretory capacity per β-cell and diabetes.

AB - ELOVL family member 6, elongation of very long-chain fatty acids (Elovl6) is a microsomal enzyme that regulates the elongation of C12-16 saturated and monounsaturated fatty acids and is related to the development of obesity-induced insulin resistance via the modification of the fatty acid composition. In this study, we investigated the role of systemic Elovl6 in the pancreatic islet and β-cell function. Elovl6 is expressed in both islets and β-cell lines. In mice fed with chow, islets of the Elovl6-/- mice displayed normal architecture and β-cell mass compared with those of the wild-type mice. However, when fed a high-fat, high-sucrose (HFHS) diet, the islet hypertrophy in response to insulin resistance observed in normal mice was attenuated and glucose-stimulated insulin secretion (GSIS) increased in the islets of Elovl6-/- mice compared with those of the wild-type mice. Enhanced GSIS in the HFHS Elovl6-/- islets was associated with an increased ATP/ADP ratio and the suppression of ATF-3 expression. Our findings suggest that Elovl6 could be involved in insulin secretory capacity per β-cell and diabetes.

KW - Fatty acid

KW - Insulin

KW - Islet

KW - Obesity

KW - Type 2 diabetes

KW - β-cell

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U2 - 10.1016/j.bbrc.2014.05.113

DO - 10.1016/j.bbrc.2014.05.113

M3 - 学術論文

C2 - 24938128

AN - SCOPUS:84904735278

SN - 0006-291X

VL - 450

SP - 318

EP - 323

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 1

ER -

Ablation of Elovl6 protects pancreatic islets from high-fat diet-induced impairment of insulin secretion

Abstract

Keywords

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