A transgenic rice seed accumulating an anti-hypertensive peptide reduces the blood pressure of spontaneously hypertensive rats

Lijun Yang; Yoshifumi Tada; Masayuki P. Yamamoto; Hui Zhao; Masaaki Yoshikawa; Fumio Takaiwa

doi:10.1016/j.febslet.2006.04.092

A transgenic rice seed accumulating an anti-hypertensive peptide reduces the blood pressure of spontaneously hypertensive rats

Lijun Yang, Yoshifumi Tada, Masayuki P. Yamamoto, Hui Zhao, Masaaki Yoshikawa, Fumio Takaiwa^*

^*Corresponding author for this work

Department of Biology

Research output: Contribution to journal › Article › peer-review

57 Scopus citations

Abstract

RPLKPW is a potent anti-hypertensive peptide designed according to the structure of ovokinin(2-7) (RADHPF). In this study, we generated transgenic rice plants that accumulate the RPLKPW peptide as a fusion protein with the rice storage protein glutelin. The engineered peptide is expressed under the control of endosperm-specific glutelin promoters and specifically accumulates in seeds. Oral administration of either the RPLKPW-glutelin fraction or transgenic rice seeds to spontaneously hypertensive rats (SHRs) significantly reduced systolic blood pressures. These results suggest the possible application of transgenic rice seed as a nutraceutical delivery system and specifically for administration of active peptides in hypertension.

Original language	English
Pages (from-to)	3315-3320
Number of pages	6
Journal	FEBS Letters
Volume	580
Issue number	13
DOIs	https://doi.org/10.1016/j.febslet.2006.04.092
State	Published - 2006/05/29

Keywords

Endosperm-specific expression
Glutelin
Hypertension
Oryza sativa
Transgenic rice

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/j.febslet.2006.04.092

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title = "A transgenic rice seed accumulating an anti-hypertensive peptide reduces the blood pressure of spontaneously hypertensive rats",

abstract = "RPLKPW is a potent anti-hypertensive peptide designed according to the structure of ovokinin(2-7) (RADHPF). In this study, we generated transgenic rice plants that accumulate the RPLKPW peptide as a fusion protein with the rice storage protein glutelin. The engineered peptide is expressed under the control of endosperm-specific glutelin promoters and specifically accumulates in seeds. Oral administration of either the RPLKPW-glutelin fraction or transgenic rice seeds to spontaneously hypertensive rats (SHRs) significantly reduced systolic blood pressures. These results suggest the possible application of transgenic rice seed as a nutraceutical delivery system and specifically for administration of active peptides in hypertension.",

keywords = "Endosperm-specific expression, Glutelin, Hypertension, Oryza sativa, Transgenic rice",

author = "Lijun Yang and Yoshifumi Tada and Yamamoto, {Masayuki P.} and Hui Zhao and Masaaki Yoshikawa and Fumio Takaiwa",

note = "Funding Information: We thank Ms. M. Utsuno, Ms. X. Wang, Ms. H. Ito and Ms. M. Yahara for technical assistance. This work was supported by a grant of development and demonstration of crop genomic breeding technology from the Ministry of Agriculture, Forestry and Fishery of Japan to F. Takaiwa and M. Yoshikawa, and Bio-oriented Technology Research Advancement Institution (BRAIN) to M.Y.",

year = "2006",

month = may,

day = "29",

doi = "10.1016/j.febslet.2006.04.092",

language = "英語",

volume = "580",

pages = "3315--3320",

journal = "FEBS Letters",

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T1 - A transgenic rice seed accumulating an anti-hypertensive peptide reduces the blood pressure of spontaneously hypertensive rats

AU - Yang, Lijun

AU - Tada, Yoshifumi

AU - Yamamoto, Masayuki P.

AU - Zhao, Hui

AU - Yoshikawa, Masaaki

AU - Takaiwa, Fumio

N1 - Funding Information: We thank Ms. M. Utsuno, Ms. X. Wang, Ms. H. Ito and Ms. M. Yahara for technical assistance. This work was supported by a grant of development and demonstration of crop genomic breeding technology from the Ministry of Agriculture, Forestry and Fishery of Japan to F. Takaiwa and M. Yoshikawa, and Bio-oriented Technology Research Advancement Institution (BRAIN) to M.Y.

PY - 2006/5/29

Y1 - 2006/5/29

N2 - RPLKPW is a potent anti-hypertensive peptide designed according to the structure of ovokinin(2-7) (RADHPF). In this study, we generated transgenic rice plants that accumulate the RPLKPW peptide as a fusion protein with the rice storage protein glutelin. The engineered peptide is expressed under the control of endosperm-specific glutelin promoters and specifically accumulates in seeds. Oral administration of either the RPLKPW-glutelin fraction or transgenic rice seeds to spontaneously hypertensive rats (SHRs) significantly reduced systolic blood pressures. These results suggest the possible application of transgenic rice seed as a nutraceutical delivery system and specifically for administration of active peptides in hypertension.

AB - RPLKPW is a potent anti-hypertensive peptide designed according to the structure of ovokinin(2-7) (RADHPF). In this study, we generated transgenic rice plants that accumulate the RPLKPW peptide as a fusion protein with the rice storage protein glutelin. The engineered peptide is expressed under the control of endosperm-specific glutelin promoters and specifically accumulates in seeds. Oral administration of either the RPLKPW-glutelin fraction or transgenic rice seeds to spontaneously hypertensive rats (SHRs) significantly reduced systolic blood pressures. These results suggest the possible application of transgenic rice seed as a nutraceutical delivery system and specifically for administration of active peptides in hypertension.

KW - Endosperm-specific expression

KW - Glutelin

KW - Hypertension

KW - Oryza sativa

KW - Transgenic rice

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U2 - 10.1016/j.febslet.2006.04.092

DO - 10.1016/j.febslet.2006.04.092

M3 - 学術論文

C2 - 16697378

AN - SCOPUS:33646539784

SN - 0014-5793

VL - 580

SP - 3315

EP - 3320

JO - FEBS Letters

JF - FEBS Letters

IS - 13

ER -

A transgenic rice seed accumulating an anti-hypertensive peptide reduces the blood pressure of spontaneously hypertensive rats

Abstract

Keywords

ASJC Scopus subject areas

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