A Lin28 homologue reprograms differentiated cells to stem cells in the moss Physcomitrella patens

Chen Li; Yusuke Sako; Akihiro Imai; Tomoaki Nishiyama; Kari Thompson; Minoru Kubo; Yuji Hiwatashi; Yukiko Kabeya; Dale Karlson; Shu Hsing Wu; Masaki Ishikawa; Takashi Murata; Philip N. Benfey; Yoshikatsu Sato; Yosuke Tamada; Mitsuyasu Hasebe

doi:10.1038/ncomms14242

A Lin28 homologue reprograms differentiated cells to stem cells in the moss Physcomitrella patens

Chen Li, Yusuke Sako, Akihiro Imai, Tomoaki Nishiyama, Kari Thompson, Minoru Kubo, Yuji Hiwatashi, Yukiko Kabeya, Dale Karlson, Shu Hsing Wu, Masaki Ishikawa, Takashi Murata, Philip N. Benfey, Yoshikatsu Sato, Yosuke Tamada^*, Mitsuyasu Hasebe

^*Corresponding author for this work

Program of Biological Science

Research output: Contribution to journal › Article › peer-review

38 Scopus citations

Abstract

Both land plants and metazoa have the capacity to reprogram differentiated cells to stem cells. Here we show that the moss Physcomitrella patens Cold-Shock Domain Protein 1 (PpCSP1) regulates reprogramming of differentiated leaf cells to chloronema apical stem cells and shares conserved domains with the induced pluripotent stem cell factor Lin28 in mammals. PpCSP1 accumulates in the reprogramming cells and is maintained throughout the reprogramming process and in the resultant stem cells. Expression of PpCSP1 is negatively regulated by its 3′-untranslated region (3′-UTR). Removal of the 3′-UTR stabilizes PpCSP1 transcripts, results in accumulation of PpCSP1 protein and enhances reprogramming. A quadruple deletion mutant of PpCSP1 and three closely related PpCSP genes exhibits attenuated reprogramming indicating that the PpCSP genes function redundantly in cellular reprogramming. Taken together, these data demonstrate a positive role of PpCSP1 in reprogramming, which is similar to the function of mammalian Lin28.

Original language	English
Article number	14242
Journal	Nature Communications
Volume	8
DOIs	https://doi.org/10.1038/ncomms14242
State	Published - 2017/01/27

ASJC Scopus subject areas

General Chemistry
General Biochemistry, Genetics and Molecular Biology
General Physics and Astronomy

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Li, C., Sako, Y., Imai, A., Nishiyama, T., Thompson, K., Kubo, M., Hiwatashi, Y., Kabeya, Y., Karlson, D., Wu, S. H., Ishikawa, M., Murata, T., Benfey, P. N., Sato, Y., Tamada, Y., & Hasebe, M. (2017). A Lin28 homologue reprograms differentiated cells to stem cells in the moss Physcomitrella patens. Nature Communications, 8, Article 14242. https://doi.org/10.1038/ncomms14242

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title = "A Lin28 homologue reprograms differentiated cells to stem cells in the moss Physcomitrella patens",

abstract = "Both land plants and metazoa have the capacity to reprogram differentiated cells to stem cells. Here we show that the moss Physcomitrella patens Cold-Shock Domain Protein 1 (PpCSP1) regulates reprogramming of differentiated leaf cells to chloronema apical stem cells and shares conserved domains with the induced pluripotent stem cell factor Lin28 in mammals. PpCSP1 accumulates in the reprogramming cells and is maintained throughout the reprogramming process and in the resultant stem cells. Expression of PpCSP1 is negatively regulated by its 3′-untranslated region (3′-UTR). Removal of the 3′-UTR stabilizes PpCSP1 transcripts, results in accumulation of PpCSP1 protein and enhances reprogramming. A quadruple deletion mutant of PpCSP1 and three closely related PpCSP genes exhibits attenuated reprogramming indicating that the PpCSP genes function redundantly in cellular reprogramming. Taken together, these data demonstrate a positive role of PpCSP1 in reprogramming, which is similar to the function of mammalian Lin28.",

author = "Chen Li and Yusuke Sako and Akihiro Imai and Tomoaki Nishiyama and Kari Thompson and Minoru Kubo and Yuji Hiwatashi and Yukiko Kabeya and Dale Karlson and Wu, {Shu Hsing} and Masaki Ishikawa and Takashi Murata and Benfey, {Philip N.} and Yoshikatsu Sato and Yosuke Tamada and Mitsuyasu Hasebe",

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T1 - A Lin28 homologue reprograms differentiated cells to stem cells in the moss Physcomitrella patens

AU - Li, Chen

AU - Sako, Yusuke

AU - Imai, Akihiro

AU - Nishiyama, Tomoaki

AU - Thompson, Kari

AU - Kubo, Minoru

AU - Hiwatashi, Yuji

AU - Kabeya, Yukiko

AU - Karlson, Dale

AU - Wu, Shu Hsing

AU - Ishikawa, Masaki

AU - Murata, Takashi

AU - Benfey, Philip N.

AU - Sato, Yoshikatsu

AU - Tamada, Yosuke

AU - Hasebe, Mitsuyasu

PY - 2017/1/27

Y1 - 2017/1/27

N2 - Both land plants and metazoa have the capacity to reprogram differentiated cells to stem cells. Here we show that the moss Physcomitrella patens Cold-Shock Domain Protein 1 (PpCSP1) regulates reprogramming of differentiated leaf cells to chloronema apical stem cells and shares conserved domains with the induced pluripotent stem cell factor Lin28 in mammals. PpCSP1 accumulates in the reprogramming cells and is maintained throughout the reprogramming process and in the resultant stem cells. Expression of PpCSP1 is negatively regulated by its 3′-untranslated region (3′-UTR). Removal of the 3′-UTR stabilizes PpCSP1 transcripts, results in accumulation of PpCSP1 protein and enhances reprogramming. A quadruple deletion mutant of PpCSP1 and three closely related PpCSP genes exhibits attenuated reprogramming indicating that the PpCSP genes function redundantly in cellular reprogramming. Taken together, these data demonstrate a positive role of PpCSP1 in reprogramming, which is similar to the function of mammalian Lin28.

AB - Both land plants and metazoa have the capacity to reprogram differentiated cells to stem cells. Here we show that the moss Physcomitrella patens Cold-Shock Domain Protein 1 (PpCSP1) regulates reprogramming of differentiated leaf cells to chloronema apical stem cells and shares conserved domains with the induced pluripotent stem cell factor Lin28 in mammals. PpCSP1 accumulates in the reprogramming cells and is maintained throughout the reprogramming process and in the resultant stem cells. Expression of PpCSP1 is negatively regulated by its 3′-untranslated region (3′-UTR). Removal of the 3′-UTR stabilizes PpCSP1 transcripts, results in accumulation of PpCSP1 protein and enhances reprogramming. A quadruple deletion mutant of PpCSP1 and three closely related PpCSP genes exhibits attenuated reprogramming indicating that the PpCSP genes function redundantly in cellular reprogramming. Taken together, these data demonstrate a positive role of PpCSP1 in reprogramming, which is similar to the function of mammalian Lin28.

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A Lin28 homologue reprograms differentiated cells to stem cells in the moss Physcomitrella patens

Abstract

ASJC Scopus subject areas

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