誘電泳動によるトラックエッチドメンブレンの微細孔内への生細胞の選択的捕捉

In this study, an insulator-based dielectrophoresis device using a track-etched membrane was developed for selective trapping of living yeast cells. A high electric field was formed in cylindrical pores of 8-μm diameter within the insulating membrane between two transparent plate electrodes. At a frequency of 20 MHz, only a living cell could be drawn into the pore and trapped strongly by dielectrophoresis. For non-spherical cells with a diameter and length of 5 µm and 14 µm, respectively, it was found that a cell longer than the pore diameter was drawn into the pores with electro-orientation if the minor axis of the cell was shorter.