β-Sitosterol and flavonoids isolated from Bauhinia malabarica found during screening for Wnt signaling inhibitory activity

Hyun Young Park; Kazufumi Toume; Midori A. Arai; Takashi Koyano; Thaworn Kowithayakorn; Masami Ishibashi

doi:10.1007/s11418-013-0762-3

β-Sitosterol and flavonoids isolated from Bauhinia malabarica found during screening for Wnt signaling inhibitory activity

Hyun Young Park, Kazufumi Toume, Midori A. Arai, Takashi Koyano, Thaworn Kowithayakorn, Masami Ishibashi^*

^*Corresponding author for this work

Department of Medicinal Resources Management, Institute of Natural Medicine

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

Screening with a cell-based luciferase assay was conducted to identify bioactive natural products which inhibit Wnt signaling activity-guided separation of an MeOH extract of Bauhinia malabarica (Caesalpiniaceae) leaves yielded five compounds, which were identified as βb-sitosterol (1), quercetin (2), 6,8-C-dimethyl kaempferol-3-O-rhamnopyranoside (3), hyperin (4), and 6,8-C-dimethyl kaempferol-3-methyl ether (5). The tested compounds 1, 3, and 5 exhibited Wnt signaling inhibitory activity, with IC₅₀ values of 0.77, 0.74, and 16.6 μM, respectively.

Original language	English
Pages (from-to)	242-245
Number of pages	4
Journal	Natural Medicines
Volume	68
Issue number	1
DOIs	https://doi.org/10.1007/s11418-013-0762-3
State	Published - 2014/01

Keywords

Bauhinia malabarica
Flavonoid
Wnt signaling pathway
β-Catenin

ASJC Scopus subject areas

Molecular Medicine

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10.1007/s11418-013-0762-3

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title = "β-Sitosterol and flavonoids isolated from Bauhinia malabarica found during screening for Wnt signaling inhibitory activity",

abstract = "Screening with a cell-based luciferase assay was conducted to identify bioactive natural products which inhibit Wnt signaling activity-guided separation of an MeOH extract of Bauhinia malabarica (Caesalpiniaceae) leaves yielded five compounds, which were identified as βb-sitosterol (1), quercetin (2), 6,8-C-dimethyl kaempferol-3-O-rhamnopyranoside (3), hyperin (4), and 6,8-C-dimethyl kaempferol-3-methyl ether (5). The tested compounds 1, 3, and 5 exhibited Wnt signaling inhibitory activity, with IC50 values of 0.77, 0.74, and 16.6 μM, respectively.",

keywords = "Bauhinia malabarica, Flavonoid, Wnt signaling pathway, β-Catenin",

author = "Park, {Hyun Young} and Kazufumi Toume and Arai, {Midori A.} and Takashi Koyano and Thaworn Kowithayakorn and Masami Ishibashi",

note = "Funding Information: Acknowledgments We are grateful to Prof. J. Nathans (Johns Hopkins University School of Medicine) for the STF/293 cells and Prof. R. T. Moon (University of Washington) for the SuperTOPFlash plasmid. This study was supported by Grants-in-aid for Scientific Research from the Japan Society for the Promotion of Science (JSPS), the Cosmetology Research Foundation, and Sekisui Chemical Innovations Inspired by Nature Research Support Program.",

year = "2014",

month = jan,

doi = "10.1007/s11418-013-0762-3",

language = "英語",

volume = "68",

pages = "242--245",

journal = "Natural Medicines",

issn = "1340-3443",

publisher = "Springer Japan",

number = "1",

}

TY - JOUR

T1 - β-Sitosterol and flavonoids isolated from Bauhinia malabarica found during screening for Wnt signaling inhibitory activity

AU - Park, Hyun Young

AU - Toume, Kazufumi

AU - Arai, Midori A.

AU - Koyano, Takashi

AU - Kowithayakorn, Thaworn

AU - Ishibashi, Masami

N1 - Funding Information: Acknowledgments We are grateful to Prof. J. Nathans (Johns Hopkins University School of Medicine) for the STF/293 cells and Prof. R. T. Moon (University of Washington) for the SuperTOPFlash plasmid. This study was supported by Grants-in-aid for Scientific Research from the Japan Society for the Promotion of Science (JSPS), the Cosmetology Research Foundation, and Sekisui Chemical Innovations Inspired by Nature Research Support Program.

PY - 2014/1

Y1 - 2014/1

N2 - Screening with a cell-based luciferase assay was conducted to identify bioactive natural products which inhibit Wnt signaling activity-guided separation of an MeOH extract of Bauhinia malabarica (Caesalpiniaceae) leaves yielded five compounds, which were identified as βb-sitosterol (1), quercetin (2), 6,8-C-dimethyl kaempferol-3-O-rhamnopyranoside (3), hyperin (4), and 6,8-C-dimethyl kaempferol-3-methyl ether (5). The tested compounds 1, 3, and 5 exhibited Wnt signaling inhibitory activity, with IC50 values of 0.77, 0.74, and 16.6 μM, respectively.

AB - Screening with a cell-based luciferase assay was conducted to identify bioactive natural products which inhibit Wnt signaling activity-guided separation of an MeOH extract of Bauhinia malabarica (Caesalpiniaceae) leaves yielded five compounds, which were identified as βb-sitosterol (1), quercetin (2), 6,8-C-dimethyl kaempferol-3-O-rhamnopyranoside (3), hyperin (4), and 6,8-C-dimethyl kaempferol-3-methyl ether (5). The tested compounds 1, 3, and 5 exhibited Wnt signaling inhibitory activity, with IC50 values of 0.77, 0.74, and 16.6 μM, respectively.

KW - Bauhinia malabarica

KW - Flavonoid

KW - Wnt signaling pathway

KW - β-Catenin

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U2 - 10.1007/s11418-013-0762-3

DO - 10.1007/s11418-013-0762-3

M3 - 学術論文

C2 - 23516045

AN - SCOPUS:84893728174

SN - 1340-3443

VL - 68

SP - 242

EP - 245

JO - Natural Medicines

JF - Natural Medicines

IS - 1

ER -

β-Sitosterol and flavonoids isolated from Bauhinia malabarica found during screening for Wnt signaling inhibitory activity

Abstract

Keywords

ASJC Scopus subject areas

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