αb-crystallin reduces ristocetin-induced soluble CD40 ligand release in human platelets: Suppression of thromboxane A2 generation

Masanori Tsujimoto, Tomoaki Doi, Gen Kuroyanagi, Naohiro Yamamoto, Rie Matsushima-Nishiwaki, Yuko Iida, Yukiko Enomoto, Hiroki Iida, Shinji Ogura, Takanobu Otsuka, Haruhiko Tokuda, Osamu Kozawa*, Toru Iwama

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Our group has previously shown that αB-crystallin (HSPB5), a small heat shock protein, inhibits human platelet aggregation by ristocetin, an activator of glycoprotein Ib/IX/V. In addition, it was demonstrated that glycoprotein Ib/IX/V activation induces soluble CD40 (sCD40) ligand release via thromboxane (TX) A2. In the present study, the effect of αB-crystallin on the ristocetin-induced sCD40 ligand release in human platelets was investigated. The ristocetin-induced release of sCD40 ligand was suppressed by αB-crystallin. In addition, αB-crystallin reduced the ristocetin-stimulated production of 11-dehydro-TX B2, a stable metabolite of TXA2. αB-crystallin did not suppress the platelet aggregation induced by U46619, a TXA2 receptor agonist. αB-crystallin had little effect on the U46619-induced phosphorylation of p38 mitogen-activated protein kinase or sCD40 ligand release. In addition, αB-crystallin failed to reduce the binding of SZ2, a monoclonal antibody against the sulfated sequence in the α-chain of glycoprotein Ib, to the ristocetin-stimulated platelets. These results strongly suggest that αB-crystallin extracellularly suppresses ristocetin-stimulated release of sCD40 ligand by inhibiting the TXA2 production in human platelets.

Original languageEnglish
Pages (from-to)357-362
Number of pages6
JournalMolecular Medicine Reports
Volume12
Issue number1
DOIs
StatePublished - 2015/07/01

Keywords

  • Platelet
  • Ristocetin
  • Soluble CD40 ligand
  • Thromboxane A
  • αB-crystallin

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Genetics
  • Oncology
  • Cancer Research

Fingerprint

Dive into the research topics of 'αb-crystallin reduces ristocetin-induced soluble CD40 ligand release in human platelets: Suppression of thromboxane A2 generation'. Together they form a unique fingerprint.

Cite this