Elucidation of transportation mechanism of NAD precursors

In this project, we tried to elucidate the transportation mechanism of NAD precursors which are expected to have beneficial effects on lifestyle diseases. To measure NAD metabolites all at once, we developed NAD metabolomics by combining HPLC and mass spectrometry. By using the methods, we found that NR, which is one of the major NAD precursors, was metabolized via novel pathway distinct from classical route.