Study on the mechanism of tissue specific diseases caused by mRNA splicing defect

We treated mammalian cells with antisense oligo for each component of spliceosome and found that all antisense oligo could inhibit splicing activity in a dose-dependent manner. Splicing inhibition by the antisense oligos decreased cell survival rate and interestingly the magnitude of the effect was different among cell lines.We also treated mouse 3T3-L1 cells with a splicing inhibitor and found that adipogenesis was defective after splicing inhibitor treatment. This inhibition was also affected the timing of addition of splicing inhibitor.