Development of a Practical SNPs Typing Method by Using Elactro-ActiveArtificial DNAs

  • 井上将彦 (Principal Investigator)
  • 藤本和久 (Co-Investigator(Kenkyū-buntansha))
  • 阿部, 肇 (Co-Investigator(Kenkyū-buntansha))

Project Details

Abstract

We have prepared new DNA probes of various structures and examined their SNP discrimination abilities for target sequences of many transition and transversion SNPs of clinical importance, with the goal of the above-mentioned practical use in mind. As a result, we have developed a new electrochemical SNP typing system composed of synthetically feasible and versatile, cheap electro-active DNA probes. This system is expected to meet the criteria of the assay protocol required by end users majoring in clinical medicines. Further improvements of our methodology are in progress for realizing an ideal SNPs sensor.
StatusFinished
Effective start/end date2006/01/012007/12/31

Funding

  • Japan Society for the Promotion of Science: ¥16,830,000.00

Keywords

  • DNAプローブ
  • SNP
  • フェロセン
  • 金電極
  • 電気化学
  • DNA Probe
  • Ferrocene
  • Au Electrode
  • Electrochemistry