Mechanism of Genome Damage Response Induced by Ultrasoun

  • Kondo, Takashi (Principal Investigator)
  • 田渕, 圭章 (Co-Investigator(Kenkyū-buntansha))
  • 趙, 慶利 (Co-Investigator(Kenkyū-buntansha))
  • TAKASAKI, Ichro (Co-Investigator(Kenkyū-buntansha))

Project Details

Abstract

A likely candidate process has been suggested to involve sonochemical activity, where reactive oxygen species mediate the generation of DNAsingle-strand breaks. Here however, we provide compelling new evidence that strongly supports a purely mechanical mechanism. Moreover, by a combination of specific assays (neutral comet tail and staining for γH2AX foci formation) we demonstrate for the first time that US exposure at even moderate intensities exhibits DNAdouble-strand breaks, through its facility to generate DNA damage across multiple cancer lines. Notably, colocalization assays highlight that ionizing radiation and ultrasound have distinctly different signatures to their respective γH2AX foci formation patterns, likely reflecting the different stress distributions that initiated damage formation.
StatusFinished
Effective start/end date2011/01/012012/12/31

Funding

  • Japan Society for the Promotion of Science: ¥3,640,000.00

Keywords

  • 超音波・DNA損傷
  • 二本鎖切断
  • リン酸化ヒストンH2AX
  • キャビテーション
  • フリーラジカル
  • DNA-PK
  • DNA損傷
  • ATM
  • ATR
  • DNA二本鎖切断