Analysis of the epitope and neutralizing capacity of human monoclonal antibodies induced by hepatitis B vaccine

We analyzed using monoclonal antibodies for neutralizing HBV, which were obtained by cell-microarray system. Epitope analysis showed that extracellular loop lesion of HBs-Ag has the antigenesity and concerns HBV-neutralization. Combination of the antibodies showed the neutralizing capacity against various genotype of HBV including G145R which is known for the escape mutant. These findings imply that HBV-vaccination is useful for the prevention of HBV-infection with various genotype or mutant HBV. Furthermore, the possibilities of antibody treatment using monoclonal antibodies or their combination for HBV-infection may be suggested.