Establishment of isolation of amnion derived stem cells

The stemness gene expression of human amnion epithelial cells （HAE) and mesenchymal cells （HAM) was compared with that of iPS cells and bone marrow derived mesenchymal stem cells （BMMSC), and was higher than BMMSC. It was suggested that HAE and HAM are closer to iPS cells than BMMSC. Drug treatment and Oct3/4 gene introduction into HAE cells made their stemness gene expression increased. Stem cells in HAE cells could be isolated according to TRA1-60 expression. HAM cells may have different differentiation potency according to their cell size.