Research output per year
Research output per year
Project Details
Abstract
The aim of this study is the development for the microchip devise with ion-channel sensor for simultaneous detection of bacteria, virus, and protein toxin. The development of detecting units for cholera toxin, MS2, and E-coli were successively achieved by constructing the antibody assembled membrane that acts as the ion-channel for ferrocyanide on the electrode surface. As the separating units, the separation of E-coli bound/unbound magnetic microbeads(1μm) was investigated using the pinched flow fractionation microchip. The determination of 5×10^2 cfu/mL E-coli was achieved by this method.
| Status | Finished |
|---|---|
| Effective start/end date | 2010/01/01 → 2011/12/31 |
Funding
- Japan Society for the Promotion of Science: ¥4,160,000.00
Keywords
- 電気化学分析
- 大腸菌
- 毒素タンパク質
- マイクロ流路
- 磁性粒子
- 細菌
- ウイルス
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Magnetic microbead-based enzyme immunoassay for ovalbumin using hydrodynamic voltammetry and fluorometric detection
Kuramitz, H., Halsall, H. B. & Heineman, W. R., 2012/06, In: Analytical Methods. 4, 6, p. 1783-1789 7 p.Research output: Contribution to journal › Article › peer-review
17 Scopus citations -
Multiplexed assay for proteins based on sequestration electrochemistry using the protein binding electroactive magnetic microbeads
Kuramitz, H., Mawatari, Y., Ikeuchi, M., Kutomi, O., Hata, N., Taguchi, S. & Sugawara, K., 2012, In: Analytical Sciences. 28, 1, p. 77-82 6 p.Research output: Contribution to journal › Article › peer-review
7 Scopus citations -
Binding assay for cholera toxin based on sequestration electr ochemistry using lactose labeled with an electroactive compound
Kuramitz, H., Miyagaki, S., Ueno, E., Hata, N., Taguchi, S. & Sugawara, K., 2011, In: Analyst. 136, p. 2373-2378Research output: Contribution to journal › Article › peer-review