Development of Simultaneous detection for Bacteria, Virus, and Toxin using Microchip with Ion-channel Sensor

  • 倉光, 英樹 (Principal Investigator)

Project Details

Abstract

The aim of this study is the development for the microchip devise with ion-channel sensor for simultaneous detection of bacteria, virus, and protein toxin. The development of detecting units for cholera toxin, MS2, and E-coli were successively achieved by constructing the antibody assembled membrane that acts as the ion-channel for ferrocyanide on the electrode surface. As the separating units, the separation of E-coli bound/unbound magnetic microbeads(1μm) was investigated using the pinched flow fractionation microchip. The determination of 5×10^2 cfu/mL E-coli was achieved by this method.
StatusFinished
Effective start/end date2010/01/012011/12/31

Funding

  • Japan Society for the Promotion of Science: ¥4,160,000.00

Keywords

  • 電気化学分析
  • 大腸菌
  • 毒素タンパク質
  • マイクロ流路
  • 磁性粒子
  • 細菌
  • ウイルス