Development of inhibitors based on short helical peptides for protein-protein interactions

  • Fujimoto, Kazuhisa (Principal Investigator)

Project Details

Abstract

I explored the effect of α-helical stabilization upon the binding of short peptides to DNAs. When a cross-linked peptide based on the homeodomain of transcription factor was titrated with a target DNA duplex, its dissociation constant (Kd) was calculated to be ca. 0.5 nM. This value was the double-digit smaller than that of the corresponding non-cross-linked peptide. The cross-linked peptide showed high substrate specificity for DNAs at the same level as the original DNA-binding protein.
StatusFinished
Effective start/end date2009/01/012010/12/31

Funding

  • Japan Society for the Promotion of Science: ¥4,290,000.00

Keywords

  • α-ヘリックス
  • タンパク間相互作用
  • 阻害剤
  • 生体高分子