Thermodynamic Analysis of Separation Behavior in Reversed-Phase Liquid Chromatography

Various types of separation media having different structural characteristics have been used. The moment analysis theory can provide some items of quantitative information about the retention equilibrium and the mass transfer kinetics in the chromatographic systems using the different packing materials. In this study, a series of new moment equations were systematically derived for the chromatographic systems using the various types of separation media by considering their shapes (spherical particle, cylindrical fiber, and flat plate) and porous structures (full-porous, partially-porous (pellicular), and non-porous). It was tried to demonstrate the usefulness of the new moment equations for evaluating the chromatographic performance of the various packing materials having the different structural characteristics as the stationary phase for the fast HPLC with a high efficiency We can use the new moment equations for the quantitative prediction of the chromatographic behavior of the various packing materials under different conditions of chromatography and evaluate their performance from the various points of view. The new moment equations are effective not only for the detailed analyses of chromatographic behavior but also for the preliminary evaluation of new types of separation media for HPLC.