Study on clinical application of a stimulation responsive gene regulation system.

I made attempts to improve our radiation responsive gene regulation system. Promoters previously constructed based on published information showed attenuated reactivity in in vivo experiments. Thus, in this study, I constructed promoters based on information about radiation responsive transcription factors actually obtained from irradiated tumor transplanted on mice. Newly obtained promoters showed reactivity to radiation equivalent to previously obtained ones. In addition, I introduced target sequences of a microRNA with tissue-specific expression into our gene of interest under the control of a radiation responsive promoter. The gene was expressed in a radiation regulation manner only in cells without the microRNA. I hope that this system will eventually lead to actual clinical application after steps of improvements.