In order to search for salivary biomarkers used for the mental health management of junior high school student, a psychosomatic stress test, a search for salivary biomarkers and an improvement on the psychology education were carried out. It was revealed that the salivary amylase activity (sAA) statistically correlate with the subjective evaluation of psychosomatic stress from a salivary test using seventy healthy subjects in first-year junior high school students for 3 weeks. Three salivary stress markers, sAA as an index of sympathetic nervous system, salivary cortisol (sCORT) as an index of endocrine system and salivary IgA (sIgA) as an index of immune system, were identified through the psychosomatic stress test. It was indicated that the salivary stress markers might be a useful screening method for preventing to be a non-attendance at school using the multiple regression analysis.