The establishment of Pacemaker cell line from human amnion delivered cells, and development of the new pacing therapy

In order to perform pacemaker cell replacement therapy using amnion delivered cells as a new therapy instead of the mechanical pacemaker implantation, we developed some methods that to purify Nanog positive cells from Human amnion delivered cells, and gene transfection of Oct4 into Human amnion delivered cells to activate into undifferentiated state. In both methods, more high rate and highly differentiated cardiomyocyte-like cells were observed. These cells showed some structures that are essential to perform as a pacemaker, ion channels that associate with automaticity, and Connexin which forms Gap junction making action potential propagate. We have showed the possibility of the pacemaker cells establishment using Human amniotic delivered cells.