Hyperdry human amniotic membrane is useful material for tissue engineering.: Physical, morphological properties, and safety as the new biological material.

  • 岡部, 素典 (Principal Investigator)
  • 二階堂敏雄. (Co-Investigator(Kenkyū-buntansha))
  • 吉田, 淑子 (Co-Investigator(Kenkyū-buntansha))
  • 野村義宏 (Co-Investigator(Kenkyū-buntansha))
  • 水田, 尚志 (Co-Investigator(Renkei-kenkyūsha))
  • 林京子 (Co-Investigator(Renkei-kenkyūsha))
  • 土岐善紀 (Co-Investigator(Renkei-kenkyūsha))
  • 相古, 千加 (Co-Investigator(Kenkyū-buntansha))

Project Details

Abstract

Human amniotic membrane (AM) has been used widely as graft biomaterial for a variety of clinical applications. But, there are some persistent problems related to the preparation, storage, and sterilization. To resolve these problems, we developed hyperdry AM (HD-AM) using far-infrared rays, depression of air, and microwaves and then sterilized by g-ray irradiation. To elucidate the benefit of HD-AM as biological materials, compare with the histological and physical properties of HD-AM with a freeze-dried AM (FD-AM) as typical freeze-dry methods, evaluate the safety of HD-AM in vivo experiment used nude mice, and demonstrate the feasibility of HD-AM transplant in pterygium. HD-AM has kept the morphological structure of epithelium and connective tissues. The water permeability and the sieving coefficient of HD-AM were significantly lower than that of FD-AM. At 18 months after transplanted, single and multilayers of HD-AM in the intraperitoneal cavity was degraded without any infiltrated cells. For clinical treatment, recurrence of pterygium and regrowth of the subconjunctival fibrosis were not observed during the 6-month follow-up periods after the surgery. It was proposed that HD-AM was a safe and effective new biological material for clinical use including treatment for recurrent pterygium.
StatusFinished
Effective start/end date2010/01/012012/12/31

Funding

  • Japan Society for the Promotion of Science: ¥4,550,000.00

Keywords

  • ヒト組織利用研究
  • ヒト乾燥羊膜
  • 海洋性コラーゲン
  • 滅菌効果
  • 減菌効果